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A seed-inventory method need to be useful for long-term storage of cultures. Cultures obtained through the ATCC should be resuscitated Based on directions. If developed in broth, the cells are pelleted by centrifugation. Resuspend in 1/twentieth the amount of fresh servicing broth, and add an equal volume of twenty% (v/v in h2o) sterile glycerol. C

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The exact definition of HEPA filter efficiency can cause some confusion in the two ASME and ISO requirements. A typical misunderstanding is that the efficiency rating applies to all particles of all sizes (e.is the commonest filter mechanism and happens once the particle is simply too large to fit between the filter fiber spaces.Returns 30-day refu

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